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Mercury Amalgam
Thimerosal
Founders
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TEST Foundation Donations and Expenses


 

Donations

Donor Year Amount
ALT Inc. 2001 $10,000
ALT Inc. 2002 $10,000

 

Expenses

Expense Amount
2001 2002
Board of Directors' Total Compensation (salary, benefits, travel expenses, etc.)

Boyd E. Haley Ph.D., Executive Director

J. Curt Pendergrass Ph.D., Assistant Director

 

$0.00

$0.00

 

$0.00

$0.00

Research (see below) $8,800.00 $8,800.00
Web site hosting $1,200.00 $1,200.00

 

Research

 

  • Inorganic mercury changes the fate of murine CNS stem cells

Sabrina Cedrola1,2, GianPaolo Guzzi2, Daniela Ferrari3, Angela Gritti3, Angelo L. Vescovi3, James C. Pendergrass4 and Caterina A.M. La Porta1,2

1Department of General Physiology and Biochemistry, Section of General Pathology, Celoria 26, Milan, Italy; 2Italian Association for Metal and Biocompatibility Research (AIRMEB), Milan, Italy; 3Institute for Stem Cell Research, Department of Biotechnology, San Raffaele Hospital, Milan, Italy; 4 Toxic Exposure Study Trust Foundation ALT, Lexington, Kentucky, USA.

Corresponding author: C.A.M. La Porta, Department of General Physiology and Biochemistry, Section of General Pathology, Celoria 26, 20133, Milan Italy. E-mail:Caterina.LaPorta@unimi.it

Submitted to FASEB Journal

ABSTRACT

Stem cells isolated from the CNS of both embryonic and adult mice can generate neurons and glia through the activation of different patterns of differentiation in dependence of exposure to appropriate epigenetic signals. On the other hand, environmental conditions might affect the proliferation, migration and differentiation of these cells. We report here, for the first time, that inorganic mercury affects the proliferative and differentiative capacity of adult neuronal stem cells (ANSC). Actually, inorganic mercury increases apoptosis in ASNC. Furthermore, in stem cell-derived astrocytes, high levels of HSP-70 occur, while the levels of GTP-b tubulin activity dramatically decrease. Interestingly, when induced to differentiate, inorganic mercury modifies morphological proprieties of astrocytes, while the neuron population is reduced. These results demonstrate that inorganic mercury produces toxicity in the ANSC-derived neuronal population and affects the biological properties of the glial-derived population.

Keywords: neuronal stem cell; inorganic mercury; HSP-70.

 

 

  • In Vitro Treatment of a Neuroblastoma Cell Line with Low Concentrations of Thimerosal Results in Apoptosis and Altered Nucleotide Binding

Kelley Kiningham, Ph.D., Research Assistant Professor, Graduate Center for Toxicology, University of Kentucky, Lexington, KY. (E-mail: kkkini0@pop.uky.edu or kiningham@Marshall.edu.

J. Curt Pendergrass, Ph.D., President, ALT, Inc. and Assistant Director, TEST Foundation, Lexington, KY (E-mail: cpender@altcorp.com)

A manuscript entitled "Mitochondrial mediated thimerosal-induced apoptosis in a human neuroblastoma cell line (SK-N-SH) A potential proapoptotic role for nuclear factor kappa binding protein (NFkB)" based in part on this work is currently being prepared by Dr. Kiningham for submission to a peer reviewed journal.

 


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